GMO lab write-ups

Hello class,

here's the format for the GMO write-up.

Write up for the lab:

1.  -Purpose

2.  -Draw the plates and label them.

3.  -Explain your group’s results (suggest why it didn’t work).  Then explain the ideal results.  Explain specifically what each was meant to show.  (Think about what a negative test would mean.)

4.  -Explain what was happening in our successful plate (#4).

5.  -I had one group that plated the PGLO- bacteria on the LB/ampicillin/arabinose plate (you know who you are...).  What would you expect to see?

AlAlso, be sure you can aswer these questions.  I will give a brief quiz on lab procedure tomorrow:

1.  What do restriction enzymes do?

2.  What do ligases do?

3.  Why was ampicillin important in this lab?

4.  What role did the arabinose play?

The homework:  Read pages 325-328 in the book.  focus on the process of selection and the use of the blue or white colonies to determine genes.  We're learning this as background.